geboy inside:
|
Wednesday, January 02, 2008 |
|
Allele-specific polymerase chain reaction diagnostic test for the functional MDR1 polymorphism in dogs
|
abstrac journal:
The major multidrug transporter P-glycoprotein (Pgp) contributes to the barrier function of several tissues and organs, including the brain. In a subpopulation of Collies and seven further dog breeds, a 4 base pair deletion has been described in the Pgp-encoding MDR1 gene. This deletion results in the absence of a functional form of Pgp and loss of its protective function. Severe intoxication with the Pgp substrate ivermectin has been attributed to the genetically determined lack of Pgp. An allele-specific polymerase chain reaction (PCR)-based screening method has been developed to detect the mutant allele and to determine if a dog is homozygous or heterozygous for the mutation. Based on this validation, the allele-specific PCR proved to be a robust, reproducible and specific tool, allowing rapid determination of the MDR1 genotype of dogs of at risk breeds using blood samples or buccal swabs.
Full text from:  sciencedirect Labels: papervet |
|
|
posted by
ali maftuh,DVM @ 9:34 AM
  |
|
|
|
|
|
